Introgression of Two Broad-Spectrum Late Blight Resistance Genes, Rpi-Blb1 and Rpi-Blb3, From Solanum bulbocastanum Dun Plus Race-Specific R Genes Into Potato Pre-breeding Lines.
Identifieur interne : 000143 ( Main/Exploration ); précédent : 000142; suivant : 000144Introgression of Two Broad-Spectrum Late Blight Resistance Genes, Rpi-Blb1 and Rpi-Blb3, From Solanum bulbocastanum Dun Plus Race-Specific R Genes Into Potato Pre-breeding Lines.
Auteurs : Elena Rakosy-Tican [Roumanie] ; Ramona Thieme [Allemagne] ; Janine König [Allemagne] ; Marion Nachtigall [Allemagne] ; Thilo Hammann [Allemagne] ; Tunde-Eva Denes [Roumanie] ; Klaudia Kruppa [Hongrie] ; Márta Molnár-Láng [Hongrie]Source :
- Frontiers in plant science [ 1664-462X ] ; 2020.
Abstract
There is a wealth of resistance genes in the Mexican wild relative of cultivated Solanum, but very few of these species are sexually compatible with cultivated Solanum tuberosum. The most devastating disease of potato is late blight caused by the oomycete Phytophthora infestans (Pi). The wild hexaploid species S. demissum, which it is able to cross with potato, was used to transfer eleven race-specific genes by introgressive hybridization that were subsequently widely used in potato breeding. However, there are now more virulent races of Pi that can overcome all of these genes. The most sustainable strategy for protecting potatoes from late blight is to pyramid or stack broad-spectrum resistance genes into the cultivars. Recently four broad-spectrum genes (Rpi) conferring resistance to Pi were identified and cloned from the sexually incompatible species S. bulbocastanum: Rpi-blb1 (RB), Rpi-blb2, Rpi-blb3, and Rpi-bt1. For this research, a resistant S. bulbocastanum accession was selected carrying the genes Rpi-blb1 and Rpi-blb3 together with race-specific R3a and R3b genes. This accession was previously used to produce a large number of somatic hybrids (SHs) with five commercial potato cultivars using protoplast electrofusion. In this study, three SHs with cv. 'Delikat' were selected and backcross generations (i.e., BC1 and BC2) were obtained using cvs. 'Baltica', 'Quarta', 'Romanze', and 'Sarpo Mira'. Their assessment using gene-specific markers demonstrates that these genes are present in the SHs and their BC progenies. We identified plants carrying all four genes that were resistant to foliage blight in greenhouse and field trials. Functionality of the genes was shown by using agro-infiltration with the effectors of corresponding Avr genes. For a number of hybrids and BC clones yield and tuber number were not significantly different from that of the parent cultivar 'Delikat' in field trials. The evaluation of agronomic traits of selected BC2 clones and of their processing qualities revealed valuable material for breeding late blight durable resistant potato. We show that the combination of somatic hybridization with the additional use of gene specific markers and corresponding Avr effectors is an efficient approach for the successful identification and introgression of late blight resistance genes into the potato gene pool.
DOI: 10.3389/fpls.2020.00699
PubMed: 32670309
PubMed Central: PMC7326066
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Introgression of Two Broad-Spectrum Late Blight Resistance Genes, <i>Rpi-Blb1</i> and <i>Rpi-Blb3</i>, From <i>Solanum bulbocastanum</i> Dun Plus Race-Specific <i>R</i> Genes Into Potato Pre-breeding Lines.</title><author><name sortKey="Rakosy Tican, Elena" sort="Rakosy Tican, Elena" uniqKey="Rakosy Tican E" first="Elena" last="Rakosy-Tican">Elena Rakosy-Tican</name><affiliation wicri:level="1"><nlm:affiliation>Plant Genetic Engineering Group, Department of Molecular Biology and Biotechnology, Babeş-Bolyai University, Cluj-Napoca, Romania.</nlm:affiliation><country xml:lang="fr">Roumanie</country><wicri:regionArea>Plant Genetic Engineering Group, Department of Molecular Biology and Biotechnology, Babeş-Bolyai University, Cluj-Napoca</wicri:regionArea><wicri:noRegion>Cluj-Napoca</wicri:noRegion></affiliation></author><author><name sortKey="Thieme, Ramona" sort="Thieme, Ramona" uniqKey="Thieme R" first="Ramona" last="Thieme">Ramona Thieme</name><affiliation wicri:level="1"><nlm:affiliation>Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Agricultural Crops, Julius Kühn-Institut, Quedlinburg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Agricultural Crops, Julius Kühn-Institut, Quedlinburg</wicri:regionArea><wicri:noRegion>Quedlinburg</wicri:noRegion><wicri:noRegion>Quedlinburg</wicri:noRegion><wicri:noRegion>Quedlinburg</wicri:noRegion></affiliation></author><author><name sortKey="Konig, Janine" sort="Konig, Janine" uniqKey="Konig J" first="Janine" last="König">Janine König</name><affiliation wicri:level="1"><nlm:affiliation>Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Horticultural Crops, Julius Kühn-Institut, Quedlinburg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Horticultural Crops, Julius Kühn-Institut, Quedlinburg</wicri:regionArea><wicri:noRegion>Quedlinburg</wicri:noRegion><wicri:noRegion>Quedlinburg</wicri:noRegion><wicri:noRegion>Quedlinburg</wicri:noRegion></affiliation></author><author><name sortKey="Nachtigall, Marion" sort="Nachtigall, Marion" uniqKey="Nachtigall M" first="Marion" last="Nachtigall">Marion Nachtigall</name><affiliation wicri:level="1"><nlm:affiliation>Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Agricultural Crops, Julius Kühn-Institut, Quedlinburg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Agricultural Crops, Julius Kühn-Institut, Quedlinburg</wicri:regionArea><wicri:noRegion>Quedlinburg</wicri:noRegion><wicri:noRegion>Quedlinburg</wicri:noRegion><wicri:noRegion>Quedlinburg</wicri:noRegion></affiliation></author><author><name sortKey="Hammann, Thilo" sort="Hammann, Thilo" uniqKey="Hammann T" first="Thilo" last="Hammann">Thilo Hammann</name><affiliation wicri:level="1"><nlm:affiliation>Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Agricultural Crops, Julius Kühn-Institut, Quedlinburg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Agricultural Crops, Julius Kühn-Institut, Quedlinburg</wicri:regionArea><wicri:noRegion>Quedlinburg</wicri:noRegion><wicri:noRegion>Quedlinburg</wicri:noRegion><wicri:noRegion>Quedlinburg</wicri:noRegion></affiliation></author><author><name sortKey="Denes, Tunde Eva" sort="Denes, Tunde Eva" uniqKey="Denes T" first="Tunde-Eva" last="Denes">Tunde-Eva Denes</name><affiliation wicri:level="1"><nlm:affiliation>Plant Genetic Engineering Group, Department of Molecular Biology and Biotechnology, Babeş-Bolyai University, Cluj-Napoca, Romania.</nlm:affiliation><country xml:lang="fr">Roumanie</country><wicri:regionArea>Plant Genetic Engineering Group, Department of Molecular Biology and Biotechnology, Babeş-Bolyai University, Cluj-Napoca</wicri:regionArea><wicri:noRegion>Cluj-Napoca</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Biological Research Centre, Jibou, Romania.</nlm:affiliation><country xml:lang="fr">Roumanie</country><wicri:regionArea>Biological Research Centre, Jibou</wicri:regionArea><wicri:noRegion>Jibou</wicri:noRegion></affiliation></author><author><name sortKey="Kruppa, Klaudia" sort="Kruppa, Klaudia" uniqKey="Kruppa K" first="Klaudia" last="Kruppa">Klaudia Kruppa</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, Hungary.</nlm:affiliation><country xml:lang="fr">Hongrie</country><wicri:regionArea>Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár</wicri:regionArea><wicri:noRegion>Martonvásár</wicri:noRegion></affiliation></author><author><name sortKey="Molnar Lang, Marta" sort="Molnar Lang, Marta" uniqKey="Molnar Lang M" first="Márta" last="Molnár-Láng">Márta Molnár-Láng</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, Hungary.</nlm:affiliation><country xml:lang="fr">Hongrie</country><wicri:regionArea>Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár</wicri:regionArea><wicri:noRegion>Martonvásár</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32670309</idno><idno type="pmid">32670309</idno><idno type="doi">10.3389/fpls.2020.00699</idno><idno type="pmc">PMC7326066</idno><idno type="wicri:Area/Main/Corpus">000129</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000129</idno><idno type="wicri:Area/Main/Curation">000129</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000129</idno><idno type="wicri:Area/Main/Exploration">000129</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Introgression of Two Broad-Spectrum Late Blight Resistance Genes, <i>Rpi-Blb1</i> and <i>Rpi-Blb3</i>, From <i>Solanum bulbocastanum</i> Dun Plus Race-Specific <i>R</i> Genes Into Potato Pre-breeding Lines.</title><author><name sortKey="Rakosy Tican, Elena" sort="Rakosy Tican, Elena" uniqKey="Rakosy Tican E" first="Elena" last="Rakosy-Tican">Elena Rakosy-Tican</name><affiliation wicri:level="1"><nlm:affiliation>Plant Genetic Engineering Group, Department of Molecular Biology and Biotechnology, Babeş-Bolyai University, Cluj-Napoca, Romania.</nlm:affiliation><country xml:lang="fr">Roumanie</country><wicri:regionArea>Plant Genetic Engineering Group, Department of Molecular Biology and Biotechnology, Babeş-Bolyai University, Cluj-Napoca</wicri:regionArea><wicri:noRegion>Cluj-Napoca</wicri:noRegion></affiliation></author><author><name sortKey="Thieme, Ramona" sort="Thieme, Ramona" uniqKey="Thieme R" first="Ramona" last="Thieme">Ramona Thieme</name><affiliation wicri:level="1"><nlm:affiliation>Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Agricultural Crops, Julius Kühn-Institut, Quedlinburg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Agricultural Crops, Julius Kühn-Institut, Quedlinburg</wicri:regionArea><wicri:noRegion>Quedlinburg</wicri:noRegion><wicri:noRegion>Quedlinburg</wicri:noRegion><wicri:noRegion>Quedlinburg</wicri:noRegion></affiliation></author><author><name sortKey="Konig, Janine" sort="Konig, Janine" uniqKey="Konig J" first="Janine" last="König">Janine König</name><affiliation wicri:level="1"><nlm:affiliation>Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Horticultural Crops, Julius Kühn-Institut, Quedlinburg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Horticultural Crops, Julius Kühn-Institut, Quedlinburg</wicri:regionArea><wicri:noRegion>Quedlinburg</wicri:noRegion><wicri:noRegion>Quedlinburg</wicri:noRegion><wicri:noRegion>Quedlinburg</wicri:noRegion></affiliation></author><author><name sortKey="Nachtigall, Marion" sort="Nachtigall, Marion" uniqKey="Nachtigall M" first="Marion" last="Nachtigall">Marion Nachtigall</name><affiliation wicri:level="1"><nlm:affiliation>Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Agricultural Crops, Julius Kühn-Institut, Quedlinburg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Agricultural Crops, Julius Kühn-Institut, Quedlinburg</wicri:regionArea><wicri:noRegion>Quedlinburg</wicri:noRegion><wicri:noRegion>Quedlinburg</wicri:noRegion><wicri:noRegion>Quedlinburg</wicri:noRegion></affiliation></author><author><name sortKey="Hammann, Thilo" sort="Hammann, Thilo" uniqKey="Hammann T" first="Thilo" last="Hammann">Thilo Hammann</name><affiliation wicri:level="1"><nlm:affiliation>Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Agricultural Crops, Julius Kühn-Institut, Quedlinburg, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Agricultural Crops, Julius Kühn-Institut, Quedlinburg</wicri:regionArea><wicri:noRegion>Quedlinburg</wicri:noRegion><wicri:noRegion>Quedlinburg</wicri:noRegion><wicri:noRegion>Quedlinburg</wicri:noRegion></affiliation></author><author><name sortKey="Denes, Tunde Eva" sort="Denes, Tunde Eva" uniqKey="Denes T" first="Tunde-Eva" last="Denes">Tunde-Eva Denes</name><affiliation wicri:level="1"><nlm:affiliation>Plant Genetic Engineering Group, Department of Molecular Biology and Biotechnology, Babeş-Bolyai University, Cluj-Napoca, Romania.</nlm:affiliation><country xml:lang="fr">Roumanie</country><wicri:regionArea>Plant Genetic Engineering Group, Department of Molecular Biology and Biotechnology, Babeş-Bolyai University, Cluj-Napoca</wicri:regionArea><wicri:noRegion>Cluj-Napoca</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Biological Research Centre, Jibou, Romania.</nlm:affiliation><country xml:lang="fr">Roumanie</country><wicri:regionArea>Biological Research Centre, Jibou</wicri:regionArea><wicri:noRegion>Jibou</wicri:noRegion></affiliation></author><author><name sortKey="Kruppa, Klaudia" sort="Kruppa, Klaudia" uniqKey="Kruppa K" first="Klaudia" last="Kruppa">Klaudia Kruppa</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, Hungary.</nlm:affiliation><country xml:lang="fr">Hongrie</country><wicri:regionArea>Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár</wicri:regionArea><wicri:noRegion>Martonvásár</wicri:noRegion></affiliation></author><author><name sortKey="Molnar Lang, Marta" sort="Molnar Lang, Marta" uniqKey="Molnar Lang M" first="Márta" last="Molnár-Láng">Márta Molnár-Láng</name><affiliation wicri:level="1"><nlm:affiliation>Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, Hungary.</nlm:affiliation><country xml:lang="fr">Hongrie</country><wicri:regionArea>Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár</wicri:regionArea><wicri:noRegion>Martonvásár</wicri:noRegion></affiliation></author></analytic><series><title level="j">Frontiers in plant science</title><idno type="ISSN">1664-462X</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">There is a wealth of resistance genes in the Mexican wild relative of cultivated <i>Solanum</i>, but very few of these species are sexually compatible with cultivated <i>Solanum tuberosum.</i> The most devastating disease of potato is late blight caused by the oomycete <i>Phytophthora infestans</i> (<i>Pi</i>). The wild hexaploid species <i>S. demissum</i>, which it is able to cross with potato, was used to transfer eleven race-specific genes by introgressive hybridization that were subsequently widely used in potato breeding. However, there are now more virulent races of <i>Pi</i> that can overcome all of these genes. The most sustainable strategy for protecting potatoes from late blight is to pyramid or stack broad-spectrum resistance genes into the cultivars. Recently four broad-spectrum genes (<i>Rpi</i>) conferring resistance to <i>Pi</i> were identified and cloned from the sexually incompatible species <i>S. bulbocastanum</i>: <i>Rpi-blb1</i> (RB), <i>Rpi-blb</i>2, <i>Rpi-blb</i>3, and <i>Rpi-bt1</i>. For this research, a resistant <i>S. bulbocastanum</i> accession was selected carrying the genes <i>Rpi-blb1</i> and <i>Rpi-blb3</i> together with race-specific <i>R3a</i> and <i>R3b</i> genes. This accession was previously used to produce a large number of somatic hybrids (SHs) with five commercial potato cultivars using protoplast electrofusion. In this study, three SHs with cv. 'Delikat' were selected and backcross generations (i.e., BC<sub>1</sub> and BC<sub>2</sub>) were obtained using cvs. 'Baltica', 'Quarta', 'Romanze', and 'Sarpo Mira'. Their assessment using gene-specific markers demonstrates that these genes are present in the SHs and their BC progenies. We identified plants carrying all four genes that were resistant to foliage blight in greenhouse and field trials. Functionality of the genes was shown by using agro-infiltration with the effectors of corresponding <i>Avr</i> genes. For a number of hybrids and BC clones yield and tuber number were not significantly different from that of the parent cultivar 'Delikat' in field trials. The evaluation of agronomic traits of selected BC<sub>2</sub> clones and of their processing qualities revealed valuable material for breeding late blight durable resistant potato. We show that the combination of somatic hybridization with the additional use of gene specific markers and corresponding <i>Avr</i> effectors is an efficient approach for the successful identification and introgression of late blight resistance genes into the potato gene pool.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">32670309</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>28</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1664-462X</ISSN><JournalIssue CitedMedium="Print"><Volume>11</Volume><PubDate><Year>2020</Year></PubDate></JournalIssue><Title>Frontiers in plant science</Title><ISOAbbreviation>Front Plant Sci</ISOAbbreviation></Journal><ArticleTitle>Introgression of Two Broad-Spectrum Late Blight Resistance Genes, <i>Rpi-Blb1</i> and <i>Rpi-Blb3</i>, From <i>Solanum bulbocastanum</i> Dun Plus Race-Specific <i>R</i> Genes Into Potato Pre-breeding Lines.</ArticleTitle><Pagination><MedlinePgn>699</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fpls.2020.00699</ELocationID><Abstract><AbstractText>There is a wealth of resistance genes in the Mexican wild relative of cultivated <i>Solanum</i>, but very few of these species are sexually compatible with cultivated <i>Solanum tuberosum.</i> The most devastating disease of potato is late blight caused by the oomycete <i>Phytophthora infestans</i> (<i>Pi</i>). The wild hexaploid species <i>S. demissum</i>, which it is able to cross with potato, was used to transfer eleven race-specific genes by introgressive hybridization that were subsequently widely used in potato breeding. However, there are now more virulent races of <i>Pi</i> that can overcome all of these genes. The most sustainable strategy for protecting potatoes from late blight is to pyramid or stack broad-spectrum resistance genes into the cultivars. Recently four broad-spectrum genes (<i>Rpi</i>) conferring resistance to <i>Pi</i> were identified and cloned from the sexually incompatible species <i>S. bulbocastanum</i>: <i>Rpi-blb1</i> (RB), <i>Rpi-blb</i>2, <i>Rpi-blb</i>3, and <i>Rpi-bt1</i>. For this research, a resistant <i>S. bulbocastanum</i> accession was selected carrying the genes <i>Rpi-blb1</i> and <i>Rpi-blb3</i> together with race-specific <i>R3a</i> and <i>R3b</i> genes. This accession was previously used to produce a large number of somatic hybrids (SHs) with five commercial potato cultivars using protoplast electrofusion. In this study, three SHs with cv. 'Delikat' were selected and backcross generations (i.e., BC<sub>1</sub> and BC<sub>2</sub>) were obtained using cvs. 'Baltica', 'Quarta', 'Romanze', and 'Sarpo Mira'. Their assessment using gene-specific markers demonstrates that these genes are present in the SHs and their BC progenies. We identified plants carrying all four genes that were resistant to foliage blight in greenhouse and field trials. Functionality of the genes was shown by using agro-infiltration with the effectors of corresponding <i>Avr</i> genes. For a number of hybrids and BC clones yield and tuber number were not significantly different from that of the parent cultivar 'Delikat' in field trials. The evaluation of agronomic traits of selected BC<sub>2</sub> clones and of their processing qualities revealed valuable material for breeding late blight durable resistant potato. We show that the combination of somatic hybridization with the additional use of gene specific markers and corresponding <i>Avr</i> effectors is an efficient approach for the successful identification and introgression of late blight resistance genes into the potato gene pool.</AbstractText><CopyrightInformation>Copyright © 2020 Rakosy-Tican, Thieme, König, Nachtigall, Hammann, Denes, Kruppa and Molnár-Láng.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Rakosy-Tican</LastName><ForeName>Elena</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Plant Genetic Engineering Group, Department of Molecular Biology and Biotechnology, Babeş-Bolyai University, Cluj-Napoca, Romania.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Thieme</LastName><ForeName>Ramona</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Agricultural Crops, Julius Kühn-Institut, Quedlinburg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>König</LastName><ForeName>Janine</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Horticultural Crops, Julius Kühn-Institut, Quedlinburg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nachtigall</LastName><ForeName>Marion</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Agricultural Crops, Julius Kühn-Institut, Quedlinburg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hammann</LastName><ForeName>Thilo</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Agricultural Crops, Julius Kühn-Institut, Quedlinburg, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Denes</LastName><ForeName>Tunde-Eva</ForeName><Initials>TE</Initials><AffiliationInfo><Affiliation>Plant Genetic Engineering Group, Department of Molecular Biology and Biotechnology, Babeş-Bolyai University, Cluj-Napoca, Romania.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Biological Research Centre, Jibou, Romania.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kruppa</LastName><ForeName>Klaudia</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, Hungary.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Molnár-Láng</LastName><ForeName>Márta</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Centre for Agricultural Research, Hungarian Academy of Sciences, Martonvásár, Hungary.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>06</Month><Day>03</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Front Plant Sci</MedlineTA><NlmUniqueID>101568200</NlmUniqueID><ISSNLinking>1664-462X</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">detached leaf assay</Keyword><Keyword MajorTopicYN="N">effectors</Keyword><Keyword MajorTopicYN="N">gene specific markers</Keyword><Keyword MajorTopicYN="N">introgression breeding</Keyword><Keyword MajorTopicYN="N">late blight field resistance</Keyword><Keyword MajorTopicYN="N">processing qualities</Keyword><Keyword MajorTopicYN="N">tuber yield</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>09</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>05</Month><Day>04</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>7</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>7</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>7</Month><Day>17</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32670309</ArticleId><ArticleId IdType="doi">10.3389/fpls.2020.00699</ArticleId><ArticleId IdType="pmc">PMC7326066</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Transgenic Res. 2012 Feb;21(1):89-99</Citation><ArticleIdList><ArticleId IdType="pubmed">21479829</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2008 Aug 06;3(8):e2875</Citation><ArticleIdList><ArticleId IdType="pubmed">18682852</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2003 Aug 5;100(16):9128-33</Citation><ArticleIdList><ArticleId IdType="pubmed">12872003</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2005 Apr;42(2):251-61</Citation><ArticleIdList><ArticleId IdType="pubmed">15807786</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Pathol. 2013 Sep;14(7):740-57</Citation><ArticleIdList><ArticleId IdType="pubmed">23710878</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Dis. 2005 Jul;89(7):692-699</Citation><ArticleIdList><ArticleId IdType="pubmed">30791237</ArticleId></ArticleIdList></Reference><Reference><Citation>J Vis Exp. 2014 Jan 03;(83):e50971</Citation><ArticleIdList><ArticleId IdType="pubmed">24430891</ArticleId></ArticleIdList></Reference><Reference><Citation>Theor Appl Genet. 2008 Mar;116(5):691-700</Citation><ArticleIdList><ArticleId IdType="pubmed">18202839</ArticleId></ArticleIdList></Reference><Reference><Citation>Theor Appl Genet. 1980 Jan;57(1):5-9</Citation><ArticleIdList><ArticleId IdType="pubmed">24302359</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2003 Dec;36(6):867-82</Citation><ArticleIdList><ArticleId IdType="pubmed">14675451</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Genet Genomics. 2003 Aug;269(5):603-11</Citation><ArticleIdList><ArticleId IdType="pubmed">12827499</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2010 Sep;23(9):1206-16</Citation><ArticleIdList><ArticleId IdType="pubmed">20687810</ArticleId></ArticleIdList></Reference><Reference><Citation>Toxicol Appl Pharmacol. 2012 Apr 15;260(2):155-61</Citation><ArticleIdList><ArticleId IdType="pubmed">22369882</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Biol Rep. 2012 Feb;39(2):1909-16</Citation><ArticleIdList><ArticleId IdType="pubmed">21667110</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Phytopathol. 2011;49:507-31</Citation><ArticleIdList><ArticleId IdType="pubmed">21663437</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Plant Biol. 2011 Jan 19;11:16</Citation><ArticleIdList><ArticleId IdType="pubmed">21247437</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2009 Jun;22(6):630-41</Citation><ArticleIdList><ArticleId IdType="pubmed">19445588</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2011 Oct;24(10):1132-42</Citation><ArticleIdList><ArticleId IdType="pubmed">21649512</ArticleId></ArticleIdList></Reference><Reference><Citation>Genetics. 2004 Oct;168(2):983-95</Citation><ArticleIdList><ArticleId IdType="pubmed">15514069</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Biotechnol J. 2019 Jun;17(6):1119-1129</Citation><ArticleIdList><ArticleId IdType="pubmed">30467980</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Rep. 2010 Oct;29(10):1187-201</Citation><ArticleIdList><ArticleId IdType="pubmed">20703881</ArticleId></ArticleIdList></Reference><Reference><Citation>Stud Mycol. 2018 Mar;89:105-115</Citation><ArticleIdList><ArticleId IdType="pubmed">29910517</ArticleId></ArticleIdList></Reference><Reference><Citation>Theor Appl Genet. 2010 Jun;121(1):117-25</Citation><ArticleIdList><ArticleId IdType="pubmed">20204320</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2005 Oct;44(2):208-22</Citation><ArticleIdList><ArticleId IdType="pubmed">16212601</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2009 Sep 17;461(7262):393-8</Citation><ArticleIdList><ArticleId IdType="pubmed">19741609</ArticleId></ArticleIdList></Reference><Reference><Citation>Theor Appl Genet. 2008 May;116(7):933-43</Citation><ArticleIdList><ArticleId IdType="pubmed">18274723</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Phytopathol. 2001;39:79-102</Citation><ArticleIdList><ArticleId IdType="pubmed">11701860</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2004 Apr;17(4):428-35</Citation><ArticleIdList><ArticleId IdType="pubmed">15077675</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Protoc. 2007;2(9):2233-44</Citation><ArticleIdList><ArticleId IdType="pubmed">17853881</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li><li>Hongrie</li><li>Roumanie</li></country></list><tree><country name="Roumanie"><noRegion><name sortKey="Rakosy Tican, Elena" sort="Rakosy Tican, Elena" uniqKey="Rakosy Tican E" first="Elena" last="Rakosy-Tican">Elena Rakosy-Tican</name></noRegion><name sortKey="Denes, Tunde Eva" sort="Denes, Tunde Eva" uniqKey="Denes T" first="Tunde-Eva" last="Denes">Tunde-Eva Denes</name><name sortKey="Denes, Tunde Eva" sort="Denes, Tunde Eva" uniqKey="Denes T" first="Tunde-Eva" last="Denes">Tunde-Eva Denes</name></country><country name="Allemagne"><noRegion><name sortKey="Thieme, Ramona" sort="Thieme, Ramona" uniqKey="Thieme R" first="Ramona" last="Thieme">Ramona Thieme</name></noRegion><name sortKey="Hammann, Thilo" sort="Hammann, Thilo" uniqKey="Hammann T" first="Thilo" last="Hammann">Thilo Hammann</name><name sortKey="Konig, Janine" sort="Konig, Janine" uniqKey="Konig J" first="Janine" last="König">Janine König</name><name sortKey="Nachtigall, Marion" sort="Nachtigall, Marion" uniqKey="Nachtigall M" first="Marion" last="Nachtigall">Marion Nachtigall</name></country><country name="Hongrie"><noRegion><name sortKey="Kruppa, Klaudia" sort="Kruppa, Klaudia" uniqKey="Kruppa K" first="Klaudia" last="Kruppa">Klaudia Kruppa</name></noRegion><name sortKey="Molnar Lang, Marta" sort="Molnar Lang, Marta" uniqKey="Molnar Lang M" first="Márta" last="Molnár-Láng">Márta Molnár-Láng</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PhytophthoraV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000143 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000143 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PhytophthoraV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:32670309
|texte= Introgression of Two Broad-Spectrum Late Blight Resistance Genes, Rpi-Blb1 and Rpi-Blb3, From Solanum bulbocastanum Dun Plus Race-Specific R Genes Into Potato Pre-breeding Lines.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:32670309" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PhytophthoraV1
| This area was generated with Dilib version V0.6.38. |  |